Inhibitory effect of procyanidin B ₂ and tannin acid on cholesterol esterase and their synergistic effect with orlistat

This study was aimed to analyze the effect of procyanidin B2 (PC) and tannin acid (TA) on activities cholesterol esterase (CEase) inhibitory mechanisms enzymatic activity. The interaction were investigated by kinetics, multi-spectroscopy methods, thermodynamics analysis, molecular docking, dynamic simulations. PC TA could bind with CEase inhibit activity enzyme in a mixed-competitive manner non-competitive manner, which verified docking simulations dynamics simulation. Also, showed synergistic inhibition orlistat. Fluorescence, UV-vis thermodynamic analysis revealed that complexes formed from inhibitors noncovalent interaction. As CD results, both decreased altering conformations CEase. might be one mechanism for its cholesterol-lowering effect.